Methodologies for target selection in structural genomics.

Prediction of the amount of secondary structure of proteins using unassigned NMR spectra: A tool for target selection in structural proteomics

With the advent of structural genomics, the need for fast structural information about unknown proteins has increased. We describe a new methodology, based on C, N and H chemical shift dispersion to predict the amount of secondary structure of unassigned proteins from their Nand/or C-edited heteronuclear single quantum coherence (HSQC) spectra. This methodology has been coded into a software ca...

Prot - 00199 - 2004 - R 1 Implications of structural genomics target selection strategies : Pfam 5000 , whole genome , and random approaches

Automatic target selection for structural genomics on eukaryotes.

A central goal of structural genomics is to experimentally determine representative structures for all protein families. At least 14 structural genomics pilot projects are currently investigating the feasibility of high-throughput structure determination; the National Institutes of Health funded nine of these in the United States. Initiatives differ in the particular subset of "all families" on...

Making Decisions for Structural Genomics

A large number of structural genomics programmes have been established worldwide with the common aim of large-scale, high-throughput protein structure determination. Due to the considerable challenges posed by the experimental methods of structural determination (primarily X-ray crystallography and nuclear magnetic resonance spectroscopy) it is important to select and prioritise candidate molec...

Target space for structural genomics revisited

MOTIVATION Structural genomics eventually aims at determining structures for all proteins. However, in the beginning experimentalists are likely to focus on globular proteins to achieve a rapid basic coverage of protein sequence space. How many proteins will structural genomics have to target? How many proteins will be excluded since we already have structural information for these or since the...